Ochou Fujin no Gensou(1940)

Butterfly, a faithful Japanese wife, waits patiently in Nagasaki for the return of her American husband, Pinkerton. She sees the Stars and Stripes fluttering atop an approaching ship and rightly surmises that Pinkerton is onboard. However, the feckless foreigner is arriving in the company of his "real" Caucasian wife, causing the heartbroken Butterfly to commit suicide. This masterpiece of Japanese silhouette animation makes the best of its source material.

(Source: The Anime Encyclopedia)